Human Tie2 (GenBank Accession No. NM_000459), a.a. 771-end with N-terminal His-tag, MW=41 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

TIE, TEK, Tyrosine Kinase With Immunoglobulin-Like And EGF-Like Domains 1

140 U/µg

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substarte per minute at pH 7.4 and 30°C.

50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate, and 2 µg/ml recombinant Tie2.

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Ramsden, J.D., et al., Endocrinology 146 (7), 2895-2902 (2005).
2. Tammela,T., et al., Blood 105 (12), 4642-4648 (2005).

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein