Recombinant human SYK Catalytic Subunit, also known as SYK, and Spleen Tyrosine Kinase, GenBank Accession No. NM_003177, a.a. 359-635(end) with a N-terminal GST-tag, expressed in Sf9 insect cells using. MW=58 kDa.

P43405

SYK, Spleen Tyrosine Kinase SYK

451 pmol/min/μg

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 0.2 mg/ml Poly(Glu:Tyr) substrate and 20 μM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Takano, T. et al. Antioxid Redox Signal. 2002 Jun,4(3):533-41.
2. Navara, C S. Curr Pharm Des. 2004,10(15):1739-44.

Avoid freeze/thaw cycles

Kinase/Cytoplasmic Tyr

Recombinant Protein