Human SRC (GenBank Accession No. NM_005417), full length with N-terminal GST-tag, MW = 86 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

c-SRC, Avian Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog, SRC1

55 pmol/min/μg

50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 0.01% Brij-35, 200 μM ATP, 2 μM Tyr2 substrate (Z-lyte kinase assay kit from Invitrogen), 1 hour at room temperature.

25 mM Tris-HCl, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Finlay, D. and Vuori, K. Cancer Res. 67 (24), 11704-11711 (2007).
2 Vielreicher, M. et al. J. Biol. Chem. 282 (46), 33623-33631 (2007).

Avoid freeze/thaw cycles

Kinase/Cytoplasmic Tyr

Recombinant Protein