SRC is a member of the nonreceptor tyrosine kinases that plays a role in many cellular functions, including cell adhesion, growth, and differentiation. SRC has been implicated in diseases such as chronic kidney disease and metastatic bone disease. The SRC Assay Kit is designed to measure SRC activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The SRC Assay Kit comes in a convenient 96-well format, with enough purified recombinant SRC enzyme, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 100 enzyme reactions.

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

c-SRC, Avian Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog, SRC1

See assay kit data sheet for detailed protocol

Up to 6 months when stored as recommended.

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. Vidal, M., et al. Differing Src signaling levels have distinct outcomes in Drosophila; 2007, Cancer Res. 67(21): 10278—10285.
2. Wan, J. and Zhuang, S. Src Family Kinases in Chronic Kidney Disease; 2017, Amer. J. Physiol. Renal Physiol.313(3): F721–F728.

Avoid multiple freeze/thaw cycles.

Kinase

Assay Kit