| 产品名称 | RIPK2, GST-tag |
| 产品货号 | 40173 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 61 kDa
Genbank #: NM_003821
Tag(s): N-terminal GST-tag
a.a: 2–299
Background: RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein which contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. RIPK2 is thought to regulate apoptosis induced by the FAS receptor pathway (1). RIPK2 has been shown to specifically interact with the CARD of ICE/caspase-1 and this interaction correlates with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (2).
Description: Human RIPK1, also known as RCK and MAP3K19, GenBank Accession No. NM_003821, a.a. 2-299, with N-terminal GST-tag, MW=61 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Synonym(s): RICK; RIP2; CARD3; CARDIAK; CCK; GIG30
Purity: ≥90%
Specific Activity: ≥15 pmol/min/μg
Assay Conditions: Assay was done in a Kinase buffer (BPS Cat. #79334) using MBP (0.2 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 minutes. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 3mM DTT, 20% glycerol
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Inohara, N. et al: RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J. Biol. Chem. 273: 12296-12300, 1998. Note: Erratum: J. Biol. Chem 273: 18675 only, 1998.
2. Thome, M. et al: Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr. Biol. 8: 885-888, 1998. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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