| 产品名称 | RIPK1 Protein, GST-Tag (Human) |
| 产品货号 | 40371 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 64 kDa
Genbank #: NM_003804
Tag(s): N-terminal GST-Th-Tag
a.a: 1-327
Background: RIPK1 or Receptor Interacting Protein Kinase 1 is a serine/threonine kinase that was originally identified as interacting with the cytoplasmic domain of FAS. RIPK1 has been deemed as an important element in the signal transduction machinery that mediates programmed cell death. RIPK1 has been shown to interact with TRADD, TRAF1 TRAF2 and TRAF3 and TRADD can act as an adaptor protein to recruit RIPK1 to the TNFR1 complex in a TNF-dependent process. TNFα is capable of activating the noncanonical NF-κB pathway, but this activation of this pathway is negatively regulated by RIPK1.
Description: Human RIPK1, also known as RCK and MAP3K19, GenBank Accession No. NM_003804, a.a. 1-327, with N-terminal GST-tag and thrombin cleavage site, MW=64 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Synonym(s): RCK, MAP3K19, receptor-interacting serine/threonine-protein kinase 1 isoform 1, receptor-interacting serine/threonine-protein kinase 1, serine/threonine-protein kinase RIP, cell death protein RIP, receptor-interacting protein 1, receptor (TNFRSF)-interact
Assay Conditions: Assay was done in a Kinase buffer (BPS Cat. #79334) using MBP (0.2 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 minutes. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 1.6 mM glutathione
Storage / Stability: At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Hsu, H. et al., Immunity 4: 387-396, 1996.
2. Kim, J Y. et al., J Cell Sci. 2011 Feb 15;124(Pt 4):647-56. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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