| 产品名称 | PKCζ (PKCzeta), GST-tag |
| 产品货号 | 40163 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 93 kDa
Genbank #: NM_002744
Tag(s): N-terminal GST-tag
a.a: full length
Background: PKCζ is an atypical isoform of the PKC family. PKCζ is found in both particulate and soluble fractions and cannot be activated by phorbol ester. Overexpression of PKCζ and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation (1). Overexpression of PKCζ also potentiates phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. PKCζ is an upstream modulator of p70S6K, an important regulator of cell proliferation (2).
Description: Recombinant human PKCζ (PKCzeta), full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Synonym(s): PRKCZ; PRKCZ; PKC2, PKCζ protein kinase c zeta
Purity: ≥75%
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Kim, SJ. Et al: Phorbol ester effects in atypical protein kinase C zeta overexpressing NIH3T3 cells: possible evidence for crosstalk between protein kinase C isoforms. Biochem Biophys Res Commun. 1997 Aug 18;237(2):336-9.
2. Romanelli, A. et al: p70 S6 kinase is regulated by protein kinase Czeta and participates in a phosphoinositide 3-kinase-regulated signalling complex. Mol Cell Biol. 1999 Apr;19(4):2921-8. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
Quality Assurance |
| 产品资料 |