| 产品名称 | PKCβ I (PKCbeta1), GST-tag |
| 产品货号 | 40158 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 102 kDa
Genbank #: X06318
Tag(s): N-terminal GST-tag
a.a: full length
Background: PKCβI is a member of the PKC family (phospholipid-dependent serine/threonine kinase) and is highly related to PKCβ II. Unlike the mature PKCβII mRNA and protein, which rapidly increase following acute insulin treatment, the PKCβI mRNA and protein levels remain unchanged (1). The stable overexpression of PKCβII, but not PKCβI, leads to insulin-stimulated glucose uptake into cells. Upon stimulation of B lymphocytes and mast cells, Syk regulates Btk, and Btk selectively regulates enzymatic activity of PKCβI. Specific regulation of PKCβI by Btk is consistent with the selective association of Btk with PKCβI (2).
Description: Recombinant human PKCβI (PKCbeta1), full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Synonym(s): PKCB; PRKCB; PRKCB2; MGC41878; PKC-beta, PKCβ1; protein kinase c beta 1
Purity: ≥75%
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Cooper, D R. et al: Ectopic expression of protein kinase CbetaII, -delta, and -epsilon, but not -betaI or -zeta, provide for insulin stimulation of glucose uptake in NIH-3T3 cells. Arch Biochem Biophys. 1999 Dec 1;372(1):69-79.
2. Kawakami, Y. et al: Regulation of protein kinase CbetaI by two protein-tyrosine kinases, Btk and Syk. Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7423-8. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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