Recombinant human serine/threonine-protein kinase PIM3 (PIM3), GenBank Accession No. NM_145478, full-length with an N-terminal GST tag and expressed in a baculovirusinfected Sf9 insect cell expression system. MW = 63 kDa.

Q86V86

serine/threonine-protein kinase PIM3

785 pmol/min/g

Varying amounts of enzyme were incubated in 5 mM MOPS, pH 7.2, 2.5 mM -glycerophosphate, 4 mM MgCl2, 2.5 mM MnCl2, 1 mM EGTA, 0.4 mM EDTA, 0.01 mM DTT, 0.05 mM ATP, 5 μg of RSK substrate and 32 μCi of [33P]-ATP was incubated at 30°C for 15 minutes. Then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol

Aqueous buffer solution

At least 6 months at –80°C.

Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Fujii, C. et al., Int. J. Cancer. 2005; 114:: 209-218.
2. Li, Y.-Y., et al., Cancer Res. 2006; 66: 6741-6747.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Kinase - Serine/Threonine

Recombinant Protein