Complex of N-terminal GST-tagged recombinant full-length human p110δ (GenBank Accession No. NM_005026), and recombinant full length, human p85α, GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system. p110δ MW=146 kDa, p85α MW=84 kDa.

PIK3CD, p110delta, Phosphoinositide-3-Kinase, Catalytic, Delta, PIK3R1, Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), GRB1, p85alpha

520 pmol/min/μg.

A unit of p110δ/p85α kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3,4,5-Trisphosphate/min at 30°C.

A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl₂, 100 uM ATP, 200 μM PI:PS substrate (BPS catalog #40560) and p110δ/p85α enzyme for 15 minutes at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform (Promega Corporation, Madison, WI).

25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.03% Tween-20, 3 mM DTT, 50% glycerol.

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Vanhaesebroeck et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94:4330.
2. Vanhaesebroeck et al. (1999) EMBO J. 18:1292.

Avoid freeze/thaw cycles

Kinase/Lipids

Recombinant Protein