| 产品名称 | PAK6, His-tag |
| 产品货号 | 40081 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 34.2 kDa
Genbank #: NM_020168
Tag(s): N-terminal His-tag
a.a: 385–680
Background: PAK6 is a Ser/Thr protein kinase that is a member of the PAK (p21-activated kinase) family that are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. PAK6 is highly expressed in the testis and prostate tissues and interacts with the androgen receptor (AR). In response to androgen, PAK6 cotranslocate into the nucleus with AR. PAK6 is weakly expressed in normal prostate epithelium but the expression is increased in primary and metastatic prostate cancer cells and is further increased in tumors that relapse after androgen deprivation therapy.
Description: Human PAK6 (GenBank Accession No. NM_020168), a.a. 385-680, MW=34.2 kDa, expressed in E. coli.
Purity: ≥95%
Specific Activity: 2.1 nmoles/min/µg
Assay Conditions: Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen). PAK5 was incubated in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 20, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction for 1 hour at RT. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm
Formulation: 25 mM Tris-HCl, pH 8, 150 mM NaCl, 10% glycerol, 5 mM DTT
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Kaur,R., et al., Prostate 68 (14), 1510-1516 (2008).
2. Hosgood,H.D. et al., Carcinogenesis 29 (10), 1938-1943(2008). Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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