Human p53 protein, also known as cellular tumor antigen p53, tumor suppressor p53, and TP53, GenBank Accession No. NM_000546, a.a. 1-393(end), expressed in a baculovirus-infected Hi5 insect cell expression system. MW = 79 kDa.

P04637

cellular tumor antigen p53, tumor suppressor p53, TP53, p53

50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

Aqueous buffer solution

>6 months at –80°C.

Useful as a kinase substrate for kinase assays, protein binding studies, or for western blotting.

1. Kastan, M.B., et al., Canc. Res. 1991; 51: 6304-6311.
2. Ryan, K.M., et al., Curr. Opin. Cell Biol. 13(3): 332-337.

Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, include carrier protein (BSA 0.1 – 0.5%).

Kinase substrate

Recombinant protein