| 产品名称 | p38α, GST-tag |
| 产品货号 | 40070 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 68 kDa
Genbank #: NM_139012
Tag(s): N-terminal GST-tag
a.a: 1-36 (end)
Background: p38α (SAPK2A) is a member of the p38 MAPK family which are activated by various environmental stresses and proinflammatory cytokines (1). The activation of p38 requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase (2). The substrates of p38 include transcription regulator ATF2, MEF2C, MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.
Description: Human p38α, also known as
MARK14, p38 MAP Kinase, and Mitogen-
Activated Protein Kinase 14, a.a. 1-360
(end), Genbank Accession No.
NM_139012 with an N-terminal GST-tag,
expressed in a Baculovirus infected Sf9
cell expression system. MW = 68 kDa.
Synonym(s): CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, MAPK14, p38
Purity: ≥90%
Supplied As: Aqueous buffer solution.
Specific Activity: 77 pmol/min/µg
Assay Conditions: Assay was performed in
kinase buffer containing 0.2 mM DTT
using P38 peptide substrate (0.1 mg/ml)
and 20 μM ATP. Reaction was done at 30
°C for 40 min. Amount of ATP transferred
was calculated using Kinase-Glo reagent
(Promega).
Formulation: 40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl, 0.04% Tween-
20, 2 mM DTT and 20% Glycerol.
Storage / Stability: >6 months at -80°C Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Johnson, G. et al., Science. 298(5600):1911-1912, 2002. Warning(s): Avoid multiple freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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