Human MEKK2, also known as MAP3K2 and MEKK2B, GenBank Accession No. NM_006609, full length, N-terminal GST-tag, MW=96.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

MEKK2B, MAP3K3, MAPKKK3, Mitogen-Activated Protein Kinase Kinase Kinase 3

18 pmol/min/µg

Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo Plus reagents (Promega Corporation, Madison, WI).

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl , 20% glycerol and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Blank, J. L. et al. J. Biol. Chem. 271:5361-5368, 1996.
2. Hammaker, D. R. et al. J. Immun. 172:1612-1618, 2004.

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein