Human JNK1-β1(K55M), also known as MAPK8 (K55M) (GenBank Accession No. NM_139046), full length with mutation of lysine55 to methionine, and N-terminal GST-tag, MW=~70 kDa, expressed in E. coli. The K55M mutation prevents ATP binding, making the kinase inactive.

JNK1b, mitogen-activated protein kinase 8, MAPK8b, Stress-Activated Protein Kinase, SAPK

inactive

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful as a substrate for kinase assays.

1. Derijard, B. et al., Cell. 76(6):1025-37 (1994).
2. Kolodziejczyk, S M. et al., Curr. Biol. 11(16):1278-82 (2001).

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein