Human Jak3 (Janus kinase 3), also known as LJAK, GenBank Accession No. NM_000215, a.a. 811-1124(end) with N-terminal His-tag, MW= 37 kDa, expressed in a baculovirus expression system.

JAK3, Janus kinase-3, Just Another Kinase 3, LJAK, jak

500 pmol/min/μg.

40 mM Tris-HCL pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr) substrate and 20 mM ATP. Reaction was done at 30°C for 45 min.

Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Kim S. et al., Cell. Signal. 21 (6), 892-898 (2009).
2. Mullighan C.G. et al., Proc. Natl. Acad. Sci. U.S.A. 106 (23), 9414-9418 (2009).

Avoid freeze/thaw cycles

Kinase/Cytoplasmic Tyr

Recombinant Protein