Human Jak2 (JH1 catalytic domain and JH2 pseudokinase domain) with W659A, W777A, and F794H mutations, GenBank Accession No. NM_004972, a.a. 536-812, with C-terminal His-Avi-Tag, expressed in an Sf9 cell expression system and enzymatically Biotin-labeled using AviTag™ technology. MW=35 kDa.

Jak2, W659A, W777A, F794H, jak

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 140 mM Imidazole and 20% glycerol.

Aqueous buffer solution.

At least 6 months at –80°C.

1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844.

2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Kinase

Receptor Tyrosine