Human Jak2 (JH1 catalytic domain and JH2 pseudokinase domain) with V617F mutation, GenBank Accession No. NM_004972, a.a. 513-1132 (end), with N-terminal GST tag, C-terminal Avi-Tag, expressed in a HEK293 cell expression system and enzymatically Biotin-labeled using AviTag™ technology.

JAK2, Janus kinase-2, Just Another Kinase 2, V617F, jh1

0.2 mg/ml

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20mM GSH and 20% glycerol.

Aqueous buffer solution

At least 6 months at –80°C. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844.
2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864.

Avoid freeze/thaw cycles.

Kinase

Receptor Tyrosine