Human Jak2, also known as Janus Kinase 2, Tyrosine-Protein Kinase JAK2, and THCYT3, GenBank Accession No. NM_004972, containing the catalytic and the pseudo kinase domains, a.a. 532-1132 (end), with N-terminal His-GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=98 kDa.

O60674

JAK2, Janus kinase-2, Just Another Kinase 2

Aqueous buffer solution.

12 pmol/min/μg

40 mM Tris-HCL pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr) substrate and 20 mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3mM DTT, 20% glycerol.

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Tono,C., et al., Leukemia 19 (10), 1843-1844 (2005).
2. Goerttler,P.S., et al., Blood 106 (8), 2862-2864(2005).

Avoid freeze/thaw cycles

Kinase/Cytoplasmic Tyr

Recombinant Protein