Human Jak2 (GenBank Accession No. NM_004972), (a.a. 808-end) with N-terminal His-tag, MW=42.5 kDa, expressed in a baculovirus expression system.

JAK2, Janus kinase-2, Just Another Kinase 2, jak, jh1

≥71%

124 pmol/min/μg.

Assay was done in Kinase buffer containing 1 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween-20 and 20% Glycerol

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Tono,C.,et al., Leukemia 19 (10), 1843-1844 (2005).
2. Goerttler,P.S., et al., Blood 106 (8), 2862-2864(2005).

Avoid freeze/thaw cycles

Kinase/Cytoplasmic Tyr

Recombinant Protein