Human insulin receptor (INSR), GenBank Accession No. NM_001079817, a.a. 999-end with an N-terminal GST-tag, expressed in a baculovirus expression system. MW=67 kDa.

P06213

INSR, insulin receptor

752 pmol/min/μg

40 mM Tris-HCL pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.1 mg/ml Alextide substrate and 20 mM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 16 mM Glutathione, 3 mM DTT and 20% glycerol

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, autophosphorylation and phosphorylation of target substrates, screening inhibitors, and selectivity profiling.

1. Zhou, P. et al. J. Pediatr. Endocrinol. Metab. 21 (4), 369-375 (2008).
2. Machorro-Lazo, M.V. et al., Gynecol. Obstet. Invest. 67 (1), 14-19 (2008).

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein