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产品名称 HPK1 Assay Kit - Kinase(s)
产品货号 79775
产品价格 现货询价，电话：010-67529703
产品规格 96 reactions
产品品牌 bpsbioscience
产品概述
产品详情
Background:
HPK1 (MAP4K1) or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family. It is a tissue-specific upstream activator of the MEKK/JNK/SAPK signaling pathway. HPK1 diminishes T cell receptor (TCR) signaling activity and T cell proliferation by phosphorylating the adaptor protein SLP-76, suggesting HPK1 could be a novel target for anti-tumor immunotherapy.
Description:
The HPK1 Assay Kit is designed to measure HPK1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Materials Required But Not Provided:
Kinase-Glo MAX (Promega #V6071) Dithiothreitol (DTT, 1 M; optional) Microplate reader capable of reading luminescence Adjustable micropipettor and sterile tips 30°C incubator
Synonym(s):
MAP4K1, Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1, Hematopoietic Progenitor Kinase 1, MAPK/ERK Kinase Kinase Kinase 1
Supplied As:
The HPK1 Assay Kit comes in a convenient 96-well format, with enough purified recombinant HPK1 enzyme, HPK1 substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Format:

CatalogNumber

Reagent

Amount

Storage

40398

HPK1

3 µg

-80°C

Avoid multiple freeze/ thaw cycles!

79334

5x Kinase assay buffer

1.5 ml

-20°C

79686

ATP (500 µM)

100 µl

-20°C

 

HPK1 Substrate (MBP; 5 mg/ml)

200 µl

-20°C

79696

96-well plate, white

1

Room Temp.

 
Storage / Stability:
Up to 6 months when stored as recommended
Application(s):
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

1. Wu P, et al., Structure, 2019, 27: 125-133.e4
2. Alzabin S, et al., J. Immunol. 2009, 182: 6187-6194
3. Jakob SM, et al., Blood,  2013, 121(20):4184-4194

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Kinase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

79775.pdf
产品资料