Human GSK3α, also known as GSK3a, glycogen synthase kinase 3 alpha, GSK3alpha, GenBank Accession No. BC027984, a.a. 2-483(end), with an N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system, MW=78 kDa.

GSK3a, glycogen synthase kinase 3 alpha, GSK3alpha

≥ 500 pmol/min/ug.

Enzyme reactions include 50 μL volumes of 1x Kinase buffer (40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA) along with 20 μM ATP, 0.2 mg/mL GSKtide substrate, 3mM DTT, and GST-GSK3α. After 45 min incubation at 30°C, 50μL of the Kinase-Glo reagent (Promega) solution was added and luminescence from remaining ATP was measured.

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM GSH, 3 mM DTT, 20% glycerol

Aqueous buffer solution

At least 6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Meijer, L. et al., Trends Pharmacol Sci. 25 (9), 471-480 (2004).
2. Patel, S. et al., Biochem Soc. Trans. 32(pt5): 803-808 (2004).

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein