| 产品名称 | GCK (MAP4K2), GST-tag |
| 产品货号 | 40107 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 125 kDa
Genbank #: NM_004579
Tag(s): N-terminal GST-tag
a.a: full length
Background: GCK is a serine/threonine protein kinase that is activated by TNFα and interacts with the TNF receptor-associated factor 2 (TRAF2). GCK is upstream of MAP kinases and particularly activates the SAPK pathway (1). Although GCK is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. A mouse protein Rab8ip that has high homology to GCK can interact with the vesicular transport protein Rab8. Based on its interaction with Rab8, it is postulated that Rab8ip/GCK may modulate secretion in response to stress stimuli (2).
Description: Human GCK, also known as MAP4K2, GenBank Accession No. NM_004579, full length, with N-terminal GST tag, MW ~125 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Synonym(s): MAP4K2, BL44, RAB8IP
Purity: ≥80%
Specific Activity: 290 pmol/min/µg
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate GCK with 0.25 mg/ml Myelin basic Protein (substrate) and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Pombo C M, et al: Activation of the SAPK pathway by the human STE20 homologue germinal centre kinase. Nature 377: 750-754, 1995.
2. Ren M, et al: In its active form, the GTP-binding protein rab8 interacts with a stress-activated protein kinase. Proc. Nat. Acad. Sci. 93: 5151-5155, 1996. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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