Human EPHA2 (GenBank Accession No. NM_004431), a.a. 596-end with N-terminal His-tag, MW=47 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

P29317

EphA2, Ephrin Type-A Receptor 2, CTPP1, ECK

170 U/mg.

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.

50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 ?M Na orthovanadate, 0.1 mM ATP, 30 ?g/ml Poly (Glu:Tyr) 4:1 substrate, and 0.1 ?g /ml recombinant EPHA2.

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol and 3.25 mM DTT.

Aqueous buffer solution

>>12 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Nakada,M., et al., Cancer Res. 64 (9), 3179-3185 (2004).
2. Kataoka,H.,et al., J. Cancer Res. Clin. Oncol. 128 (7), 343-348 (2002).

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein