Human CSK (GenBank Accession No. NM_004383), full length with N-terminal GST-tag, MW= 80 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

CSK, C-terminal Src kinase

100 U/mg.

One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.

50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.1 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate, and 1 µg/ml recombinant CSK.

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, 10 mM reduced glutathione, and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Manjarrez-Orduño N, et al. Nat Genet. 2012 Nov,44(11):1227-30.
2. de la Puerta ML, et al. PLoS One. 2013,8(1):e54569.

Avoid freeze/thaw cycles

Kinase/Cytoplasmic Tyr

Recombinant Protein