Rat CERK, also known as ceramide kinase, GenBank Accession No. NM_00113486, 1-531 (end) with C-terminal Flag tag, MW= 61 kDa, expressed in baculovirus expression system.

ceramide kinase

≥20 pmole/min/μg

CERK activity was measured at 37^oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega, Madison, WI).

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 100ug/ml flag peptide

Aqueous buffer solution

At least 6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Sugiura, M. et al., JBC. 277, 23294-23300 (2002)

2. Majjalieh, S.M., et al., JBC. 264, 14354-14360 (1989)

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Kinase

Protein