Human ceramide kinase (CERK), GenBank Accession No. NM_022766, a.a. 1-537(end) with C-terminal FLAG-tag, MW= 60 kDa, expressed in a baculovirus expression system.

Q8TCT0

CERK, Ceramide Kinase, Lipid kinase 4, LK4, Acylsphingosine Kinase

≥90 pmole/min/µg

A unit of ceramide kinase activity is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.

CERK activity was measured at 37°C for 25 minutes in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl₂, 10 mM MgCl₂, 2 mM DTT, 5 μM ATP) using a beta-Octylglucoside mixed micellar assay with 100 μM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo^® Luminescent Kinase Assay Platform (Promega, Madison, WI).

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% Glycerol and 100 μg/mL Flag peptide.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Mitra,P. et al., FEBS Lett. 581 (4), 735-740 (2007)
2. Kim,T.J. et al., Biochem. Biophys. Res. Commun. 342 (2), 611-617 (2006)

Avoid freeze/thaw cycles

Kinase/Lipids

Recombinant Protein