Cyclin-dependent kinase 7 (CDK7) assembled with Cyclin H and MAT1 comprises an important sub-component of the transcription factor TFIIH. It is known to have dual roles, which include transcription regulation via phosphorylating the C-terminal domain (CTD) of RNA polymerase II as well as controlling cell cycle progression as a CDK activating kinase (CAK). The CDK7 Assay Kit is designed to measure CDK7/Cyclin H/MAT1 activity for screening and profiling applications, using ADP-Glo® as a detection reagent.

Q24216

Cyclin-dependent kinase 7, cyclin dependent kinase 7, CDK7, CDK-7.

The CDK7 Assay Kit comes in a convenient 96-well format, with enough purified recombinant CDK7/Cyclin H/MAT1 enzyme, CDK substrate peptide, ATP and kinase assay buffer for 100 enzyme reactions.

See assay kit data sheet for detailed protocol.

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Uzma A. et al., Nature Review Drug Discovery 14:130-146 (2015)

Avoid freeze/thaw cycles.

Kinase

Assay Kit