Complex of N-terminal Histagged recombinant full-length human CDK6 (GenBank Accession No. X66365), and N-terminal His tagged, recombinant full length, Cyclin D3. (GenBank Accession No. M90814). Coexpressed in a Baculovirus infected Sf9 cell expression system. CDK6 MW=38 kDa, Cyclin D3 MW=33 kDa.

Q00534 (CDK6); P30281 (Cyclin D3)

Cyclin-Dependent Kinase 6, Cell Devision Kinase 6, CDK6, Cyclin D3, CCD3, G1/S-Specific Cyclin D3, CCND3

12 pmol/min/µg

Assay was done in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 M ATP at 30°C for 60 min. The amount of ATP transferred was calculated using Kinase- Glo reagent (Promega).

40 mM Tris-HCl, 110 mM NaCl, 2.2 mM KCl, 300 mM Imidazole, 3 mM DTT and 20% glycerol

Aqueous buffer solution

At least 6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Gauduchon J. et al., Int J Cancer. 2008 May 1,122(9):2130-41.
2. Faast R. et al., Oncogene. 2004 Jan 15,23(2):491-502.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended.

Kinase/Serine-Threonine

Recombinant Protein