| 产品名称 | CDK5/p25, GST-tag |
| 产品货号 | 40105 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 60 kDa (CDK5), 50 kDa (p25)
Genbank #: NM_004935, NM_003885
Tag(s): N-terminal GST-tag
a.a: CDK5 1–292 (end), p25 99–307 (end)
Background: CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).
Description: Complex of recombinant full length human CDK5 (GenBank Accession No. NM_004935), a.a. 1-292 (end) with N-terminal GST-tag and p25 (GenBank Accession No. NM_003885), a.a. 99-307 (end) with N-terminal GST-tag. Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK5 MW=60 kDa, p25 MW=50 kDa.
UniProt Q00535 (CDK5), Q15078 (p35)
Synonym(s): Cyclin-Dependent Kinase-5, CDK5: PSSALRE
p25: CDK5R1; CDK5P35, CDK5R, NCK5A, p23, p35, p35nck5a Specific Activity: 312 pmol/min/μg
Assay Conditions: Assay was done in a Kinase buffer using Histone H1 (BPS cat. #52043) as a substrate with 20 μM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 0.04% Tween-20, and 20% glycerol
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Tang, D. et al: Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators. Prog Cell Cycle Res. 1996;2:205-16.
2. Dhavan, R. et al: A decade of CDK5. Nat Rev Mol Cell Biol. 2001 Oct;2(10):749-59. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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