Recombinant full-length human CDK2 (GenBank Accession No. NM_001798), a.a. 2-298 (end) with N-terminal GST-tag, MW = 61 kDa, and CyclinA2 (GenBank Accession No. NM_001237), a.a. 174-432 (end) with N-terminal His-GST-tags, MW = 75 kDa. Both proteins were coexpressed in a baculovirus Sf9 insect cell expression system.

P24941 (CDK2), Q13825 (CyclinA2)

Cyclin-Dependent Kinase 2, Cell Devision Kinase 2, CDK2, Cyclin A2, CCN1, CCNA2

390 pmol/min/µg

Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/ml) substrate and 20 µM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Brown NR., et al., Cell Cycle. 2007 Jun 1,6(11):1350-9.
2. Hara KT., et al., Dev Biol. 2005 Oct 1,286(1):102-13.

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein