Complex of recombinant full-length human CDK2 (GenBank accession No. NM_001798), a.a. 1-298 (end), MW = 34 kDa with no tag, and CyclinA2 (GenBank accession No. NM_001237), a.a. 174-432 (end) with N-terminal His-GST-tags, MW = 57 kDa. Both were co-expressed in a baculovirus Sf9 insect cell expression system.

P24941 (CDK2), P20248 (CyclinA2)

Cyclin-Dependent Kinase 2, Cell Devision Kinase 2, CDK2, Cyclin A2, CCN1, CCNA2

203 pmol/min/µg

Assay was performed in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo^® reagent (Promega).

20 mM NaH₂PO₄/Na₂HPO₄, pH 8.0, 400 mM NaCl, 20 mM glutathione, 10% glycerol, 2 mM DTT, and 1 mM EDTA

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Brown NR., et al., Cell Cycle. 2007 Jun 1,6(11):1350-9.
2. Hara KT., et al., Dev Biol. 2005 Oct 1,286(1):102-13.

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein