Human CDK1/CyclinB1 complex, also known as CDC and CCNB1, CCNB respectively. GenBank Accession Nos. NM_001786 and NM_031966 respectively, full-length with N-terminal GST-tags on both proteins, expressed in a baculovirus-infected Sf9 cell expression system. MW = 60 kDa (CDK1) MW = 75 kDa (CyclinB1).

CDK1: P06493; CyclinB1: P14635

Cyclin-dependent kinase 1, CDC and CCNB1, CCNB respectively

90 pmol/min/μg Assay Conditions: Assay was done in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 M ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.

Aqueous buffer solution

At least 6 months at -80°C

Useful for studying enzyme kinetics and screening inhibitors.

1. Vantieghem, A. et al., J. Biol. Chem. 2002; 277(40): 37718-37731.

2. Rigas, A.C. et al., Oncogene. 2007; 26(55): 7611-7619.

Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Kinase-Serine/Threonine

Recombinant Protein