c-Raf (Raf-1; proto-oncogen c-Raf) is a member of the Raf (rapidly accelerated fibrosarcoma) kinase family that plays an important role in regulating the MEK and ERK pathways. The c-Raf Kinase Assay Kit is designed for measuring c-Raf activity for screening and profiling applications.

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

The c-Raf Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant c-Raf enzyme, c-Raf substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Up to 6 months when stored as recommended.

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Nakamura, J.L. Expert Opin. Ther. Targets 11(4):463-472 (2007)

Kinase

Assay Kit