Human recombinant BRSK2 (GenBank Accession No. NM_003957), full length with N-terminal His-tag, MW=75 kDa, expressed in expressed in a baculovirus expression system.

BRSK2, BR Serine/Threonine Kinase 2, SADA, STK29, SAD1, Brain-Selective Kinase 2

412 pmoles/min/µg

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (Ser/Thr 21, Invitrogen) at room temperature for 1 hour.

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, and 10% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Bright N.J. et al., J. Biol. Chem. 283 (22), 14946-14954 (2008).
2. Guo Z. et al., Biochem. Biophys. Res. Commun. 347 (4), 867-871 (2006).

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein