产品名称 | Aurora A Protein Kinase, His-Tag (Sf9-derived) |
产品货号 | 40004 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | 10 µg |
产品品牌 | bpsbioscience |
产品概述 | |
产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 50 kDa
Genbank #: NM_003600
Tag(s): N-terminal His-tag
a.a: 1-403 (end)
Description: Human Aurora Kinase A also
known as STK15, STK6, BTAK, HARK1,
ARK1, and AURA, GenBank Accession
No. NM_003600, a.a. 1-403 (end) with Nterminal
His-tag, expressed in a
Baculovirus infected Sf9 cell expression
system, MW=50 kDa.
Synonym(s): Aurora Kinase A, STK15, STK6, BTAK, HARK1, ARK1, AURA
Specific Activity: 45 pmol/min/µg
Assay Conditions: Assay was done in
Kinase buffer containing 2 mM DTT using
KEMPtide substrate (0.2 mg/ml) and 10
μM ATP. Reaction was done at 30oC for
45 min. Amount of ATP transferred was
calculated using ADP-Glo reagent
(Promega).
Formulation: 40 mM Tris pH 8.0, 110
mM NaCl, 2.2 mM KCl, 0.04% Tween-20,
20% glycerol, and 3 mM DTT.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Ouchi,M. et al., J. Biol Chem. 279 (19), 19643-19648 (2004).
2. Kunitoku, N. et al., Dev. Cell 5 (6), 853-864 (2003). Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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产品资料 |