Human Ten-eleven translocation 3 protein (TET3), also known as methylcytosine dioxygenase TET3, GenBank Accession # NM_001287491, a.a. 824-1795 (end) with N-terminal FLAG-tag and C-terminal His-tag. MW = 108 kDa, expressed in Sf9 cells via a baculovirus expression system.

methylcytosine dioxygenase TET3

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, and 140 mM Imidazole

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Tian-Peng, G., et al., Nature. 2011;477: 606-610.

2. Abdel-Wahab, O., et al., Blood. 2009; 114: 144-147.

Demethylase

Recombinant Protein