TET1 protein (Human Ten-eleven translocation 1), methylcytosine dioxygenase TET1, Leukemiaassociated protein with a CXXC domain, CXXC-type zinc finger protein 6, GenBank Accession # NM_030625, a.a. 1418-2136(end) with N-terminal FLAG-tag, MW = 80 kDa. This TET1 protein is expressed in Sf9 cells via a baculovirus expression system.
| 产品名称 | TET1, FLAG-tag - Enzymes - Demethylase(s) |
| 产品货号 | 50161-1 50161-2 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg 50 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 80 kDa
Genbank #: NM_030625
Tag(s): N-terminal FLAG-tag
a.a: 1418-2136
Description: UniProt Q8NFU7
Synonym(s): Ten-eleven translocation 1 protein (TET1), methylcytosine dioxygenase TET1, Leukemiaassociated protein with a CXXC domain, CXXC-type zinc finger protein 6, tet1 protein
Assay Conditions: TET enzyme was
combined with a TET methylatedsubstrate
in assay buffer for 90 minutes.
A fluorescent-conjugated antibody that
recognizes hydroxymethylated product
was added and fluorescence was read at
excitation: 530 nm and emission 590 nm.
Formulation: 40 mM Tris-HCl, pH 8.0, 110
mM NaCl, 2.2 mM KCl, 0.04% Tween-20,
20% glycerol, 80 μg/ml FLAG peptide
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Tahiliani, M., et al., Science. 2009; 324(5929): 930-935.
2. Guo, J.U., et al., Cell. 2011; 145(3): 423- 434. Warning(s): Avoid
freeze/thaw cycles. Storing diluted enzyme is
not recommended, if necessary, use carrier
protein (BSA 0.1 – 0.5%).
Scientific Category: DNA Demethylase
Product Type: Recombinant protein
Data shown is lot-specific. Contact us for specific information on other lots.
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