Human PHF8 also known as KDM7B (lysine-specific demethylase 7B), JHDM1F, and KIAA1111, GenBank Accession No. NM_001184896, full-length (a.a. 2-1060(end)), with N-terminal FLAG-tag, MW=120 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

Q9UPP1

KDM7B, JHDM1F, PHD finger protein 8, ZNF422

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 μg/ml FLAG peptide

Aqueous buffer solution

At least 6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Liu, W. et al., Nature. 2010; 466(7305): 508-512.
2. Zhu, Z. et al., Cell Res. 2010; 20(7): 794-801.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Demethylases

Recombinant Protein