The PHF8 Homogeneous Assay Kit is designed to measure the activity of PHF8 for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 on histone H3. The PHF8 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified PHF8 for 384 enzyme reactions. The key to the PHF8 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing PHF8 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

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KDM7B, JHDM1F, PHD finger protein 8, ZNF422, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen

The PHF8 (KDM7B) Homogeneous Assay Kit comes in a convenient AlphaLISA® format with primary antibody 17-4, FLAG-tagged PHF8, and assay buffers for 384 reactions.

Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and HTS applications.

Horton J.R., et al. Nature Structural & Molecular Biology 2010; 17(1):38-43.

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Avoid freeze/thaw cycles.

Demethylase

Assay Kit