The JMJD2D Chemiluminescent Kit is designed to measure activity of JMJD2D for screening and profiling applications. The key to the JMJD2D Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2D enzyme is incubated with a sample containing assay buffer for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

KDM4D, JMJD2D

Kit comes in a convenient format, with 8-well strips pre-coated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified JMJD2D for 100 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

COMPONENTS:

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Whetstine, J.R. et al., Cell 2006; 125: 467.

The buffer in this kit was reformulated in May of 2015 with a different reducing agent to improve assay performance. The old formulation can still be purchased upon special request.

Avoid freeze/thaw cycles.

Demethylase

Assay Kit