Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015) (a.a. 1-500) with C-terminal FLAG-tag, MW=57 kDa, expressed in Sf9 cells using a Baculovirus expression system.

O94953

JHDM3B, KDM4B

≥0.23 pmole/min/μg

10µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/mL FLAG peptide and 20% glycerol

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Fodor, B.D., et al. (2006). Genes Dev. 20(12): 1557–1562.
2. Beyer S., et al. (2008). J. Biol. Chem. 283(52):36542-52.

Avoid freeze/thaw cycles.

Demethylase

Recombinant Protein