Human JMJD1A (also known as KDM3A or JHDM2A), GenBank Accession No. NM_018433, a.a. 2-1321 (end), with N-terminal FLAG-tag, MW=148.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q9Y4C1

JMJD1A, KDM3A, JHDM2A

≥0.09 pmole/min/μg

10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α- ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 1 mM TCEP, 0.5 µM biotinylated peptide substrate, and JMJD1A (20 – 100 ng) added to the wells. Add antibody against mono-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ng/µl FLAG peptide, and 10% glycerol.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Du ZM. et al., PLoS One.; 6(4) : e19137 (2011).
2. Guo X. et al., Neoplasma.; 52(2): 153-157 (2011).

Application Reference(s):
A scintillation proximity assay for histone demethylases (2014)

Avoid freeze/thaw cycles.

Demethylase

Recombinant Protein