The JARID1B Homogeneous Assay Kit is designed to measure activity of the JARID1B for screening and profiling applications. The key to the JARID1B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JARID1B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

JARID1B, PLU-1, KDM5B, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen

The JARID1B Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1B for 384 enzyme reactions.

Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.

COMPONENTS:

See assay kit data sheet for detailed protocol.

>6 months at –80°C

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Lahoud, M.H., et al. Genome Res 11 (8): 1327–34.

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Avoid freeze/thaw cycles.

Demethylase

Assay Kit