Human JARID1A protein, also known as KDM5A and RBBP2, GenBank Accession No. NM_001042603, a.a. 2-1090 with N-terminal His-Avi-tags and C-terminal FLAG-tag, MW= 128 kDa, expressed in Sf9 cells via a baculovirus expression system.
| 产品名称 | JARID1A Protein |
| 产品货号 | 50155 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 128 kDa
Genbank #: NM_001042603
Tag(s): N-terminal His-Avi-tags, C-terminal FLAG-tag
a.a: 2–1090
Description: UniProt P29375
Synonym(s): KDM5A, RBBP2, JARID1A
Specific Activity: ≥0.035 pmol/min/µg
Assay Conditions: 10µl reaction mix
containing assay buffer with 20 mM
HEPES (pH 7.4), 50 mM NaCl, 1mM
TCEP, 500 µM alpha-ketoglutarate, 25 µM
iron, 2mM ascorbic acid, 0.01% Tween-20,
0.5 µM biotinylated peptide substrate, and
Jarid1A (20 – 100ng) added to the wells.
Add antibody against di-methylated K4
peptide. Incubate for 30 min, then
Streptavidin-conjugated secondary
antibody followed by Alpha Screening
detection.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 80 µg/ml FLAG peptide, and 20% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C. Application(s): Useful for the study of JARID1A enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Roesch, A., et al. (2005). Mod Pathol. 18(9):1249-57. Application Reference(s):
Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Demethylase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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