| 产品名称 | Sirtuin 5, GST-tag - Enzymes - Deacetylase(s) |
| 产品货号 | 50016 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 100 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 59.8 kDa
Genbank #: NM_012241
Tag(s): N-terminal GST-tag
a.a: full length
Description: Human Sirtuin 5 (GenBank Accession No. NM_012241), full length with N-terminal GST-tag, MW= 59.8 kDa, expressed in an E. coli expression system.
UniProt Q9NXA8
Synonym(s): Sirtuin5, SIRT5
Specific Activity: ≥2.0 pmole/min/µg
Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA, 200 µM SIRT5 substrate 5 (Catalog #50401), 500 µM NAD+ and Sirtuin 5 (15.6 - 1000 ng). Incubation condition: 30 min at 37°C followed by
treatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione and 10% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Mahlknecht, U. et al., (2006) Cytogenet.
Genome Res. 112, 208-212.
2. Frye, R.A. (2000) Biochem. Biophys. Res. Commun. 273, 793-798. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Deacetylase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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