Human Sirtuin 3 (GenBank Accession No. NM_012239), a.a. 102-399 with N-terminal GST-tag, MW= 59 kDa, expressed in an E. coli expression system.

Q9NTG7

Sirtuin3, SIRT3

3.7 pmole/min/µg

25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM HDAC substrate 1 (Catalog #50032), 500 µM NAD+ and Sirtuin 3 (31 - 2000 ng). Incubation condition: 30 min at 37°C followed by treatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Scher, M.B. et al., Genes Dev. 21 (8), 920-928 (2007)
2. Bellizzi, D. et al., Genomics 89 (1), 143-150 (2007)

Avoid freeze/thaw cycles.

Deacetylase

Recombinant Protein