Trichostatin A (TSA) is an antifungal antibiotic with cytostatic and differentiating properties in mammalian cell culture.

TSA

≥98% by HPLC

TSA inhibited proliferation of eight breast carcinoma cell lines with mean ±SD IC50 of 124.4 ± 120.4 nM (range of 26.4 nM –308.1 nM). HDAC inhibitory activity of TSA was similar in all cell lines with mean ± SD IC50 of 2.4 ± 0.5 nM (range of 1.5 nM–2.9 nM), and TSA treatment resulted in pronouncing histone H4 hyperacetylation. TSA inhibited HDAC3 with IC50 of 0.9 nM using the BPS Bioscience Fluorogenic HDAC Assay Kit (Cat. #50033).

Soluble to 10 mM in ethanol and to 50 mM in DMSO

White to beige powder.

Store at or below –20°C. Solid form is stable at least 12 months from date of receipt, when stored as directed. Do not store aqueous solutions for more than one day.

Festuccia, C. et al. Endocr Relat Cancer 2009;16: 401-413

HDAC

Inhibitor