Human HDAC7 , GenBank Accession No. AY302468, a.a. 518-end, with N-terminal GST-tag, MW= 78 kDa, expressed in baculovirus expression system.

Q8WUI4

histone deacetylase 7, HDAC-7

≥15,000 pmol/min/µg.

One U =1 pmol of acetyl group removed/min/µg of enzyme

25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC7 (0.16 - 10 ng). Incubation condition: 30 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 20 mM glutathione, and 10% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Chakraborty S. (2006) J.Biol.Chem. 281(46):35070-80.
2. Wolfgang, F. (2001) J. Biol. Chem. 276(38): 35826-35835.

Application References:
1. Impact of binding mechanism on selective inhibition of histone deacetylase isoforms (2017) 
2. In Silico and in Vitro Interactions between Short Chain Fatty Acids and Human Histone Deacetylases (2017)
3. Structural Characterization of the SMRT Corepressor Interacting with Histone Deacetylase (2017)
4. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)
5. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
6. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013)
7. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013)
8. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)

Avoid freeze/thaw cycles.

Deacetylase

Protein